Figure 6

Effect of miR-146a inhibitors and mimics upon IL-1β-induced IL-6 and IL-8 release. HASM cells were electroporated in the presence of buffer (A-C), control inhibitor or miR-146a inhibitor (A), control mimic or miR-146a mimic (B) and control siRNA or an siRNA targeted at IL-6 (C). Cells were then exposed to vehicle control or 1 ng/ml IL-1β and the release of IL-6 and IL-8 was measured by ELISA at 24 h. The results are mean ± SEM of three independent experiments where * p < 0.05 versus IL-1β-stimulated cells.