Figure 10

Effect of miR-146a mimics upon IL-1β-induced IL-6 and IL-8 mRNA expression. HASM cells were electroporated in the presence of buffer, control mimic or miR-146a mimic. Cells were then exposed to vehicle control or 1 ng/ml IL-1β and the expression of IL-6 and IL-8 mRNA was measured by qRT-PCR at 24 h. The results are the mean ± SEM of three independent experiments. Panel A shows the absolute increases in IL-6 and IL-8 mRNA whilst the results in panel B are expressed as a % compared to IL-1β stimulation (100%).