Figure 1

Recovery of verapamil-reduced alveolar fluid clearance (AFC) by K⁺channel openersin vivo. (A) Verapamil intratracheal application reduces alveolar fluid clearance. Verapamil (100 μM) was intratracheally delivered to mouse lung. Average AFC values in the absence of drugs (Control), in the presence of amiloride (Amiloride), verapamil (Verapamil), and both (Amiloride+Verapamil). Unpaired two-sample two-tailed Student t-test. *P < 0.05 and **P < 0.01 when compared with Control. n = 4-15. (B) Amiloride-sensitive (AS) AFC. The mean and SE values were computed as described in Methods. Unpaired two-sample two-tailed Student t-test. **P < 0.01. n = 12-15. (C) Effects of K⁺ channel openers on basal AFC. Unpaired two-sample two-tailed Student t-test. n = 5-15. (D) K⁺ channel openers restore verapamil-reduced AFC. AFC values were measured for Verapamil (100 μM) alone, + Pyrithione-Na (1 mM), + 1-EBIO (1 mM), and +Minoxidil (0.6 mM). The dashed line indicates the Control level. Unpaired two-sample two-tailed Student t-test. *P < 0.05 vs Verapamil alone. n = 4-12.