Figure 3

GC frass-induced experimental allergic asthma in wild type and PAR-2-deficient mice. Balb/c wild type and PAR-2-deficient mice were challenged by intratracheal inhalation on day 0, 7, and 14 with PBS or GC frass (40 μg). On day 17, mice were anesthetized and acetylcholine was injected after establishment of a stable airway pressure. In all cases the data are expressed as mean ± SEM and represent 4-5 mice per group and statistical significance determined by ANOVA. A. AHR was measured as airway pressure time index (APTI) in cm-H₂O × sec ^-1 (compared to PBS * p < 0.001, compared to GC frass **p = 0.014). B. Serum IgE levels were analyzed by ELISA (compared to PBS *p = 0.011, compared to GC frass **p = 0.024). C-F. Lungs from the mice were excised; cells dissociated and maintained in a single suspension culture for 3 days in the presence of Con A (10 μg/ml). C. IL-13 levels (compared to PBS *p < 0.001, **p = 0.004, compared to GC frass ***p = 0.041). D. IL-5 levels (compared to PBS *p < 0.001, compared to GC frass **p = 0.004). E. IL-17A levels F. IFNγ levels (compared to PBS *p < 0.05).