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Figure 3 | Respiratory Research

Figure 3

From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis

Figure 3

Effect of 24 h CFTR inhibition and 72 h CFTR inhibition with CFTR inh -172 (20 μM) on membrane cholesterol content. A) Representative traces of membrane cholesterol determination in 9/HTEo-cells after treatment with the CFTR inhibitor CFTRinh-172 for either 24 h or 72 h with fresh inhibitor placed on cells every 24 h or cells with no treatment (NT). B) Quantification of responses between cells with no treatment (NT) and cells with acute (24 h) or chronic (72 h) CFTR inhibition. Responses are reported relative to NT response (response ratio) to indicate the fold difference in response. Error bars represent SEM, n = 4 for each. Significance determined by ANOVA relative to NT samples. *p < 0.001.

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