Figure 5

Time-dependent effect ofS. aureussupernatant on the ion and water content. (A) We observed a significant (*, p < 0.05) time-dependent increase in sodium concentration and decrease in sulfur and chloride concentrations in the cell cytoplasm. (B) In the secretory granules, S. aureus supernatant incubation induced a significant increase in sulfur and potassium concentrations (*, p < 0.05; **, p < 0.01) and in parallel a significant (*, p < 0.05) decrease in chloride concentration. (C) The water content was significantly decreased in a time-dependent way by S. aureus supernatant in the cytoplasm and (D) in the secretory granules (*, p < 0.05). Data represent the mean ± SEM from 36 to 65 cytoplasmic areas or secretory granules from 14 to 21 cells.