Effect ofS. aureussupernatant on cAMP-mediated chloride efflux. (A) Time-dependent effect of S. aureus supernatant incubation on cAMP-mediated chloride efflux. We observed a significant (p < 0.01) time-dependent decrease in chloride efflux when cells were incubated with 2% S. aureus supernatant. This decrease became significant as soon as after 1 hour of incubation with 2% VF. (B) CFTRinh172 significantly decreased the chloride efflux compared to control (**, p < 0.01) and this decrease was similar to the decrease induced by S. aureus supernatant. Data represent the mean ± SEM of 3 different experiments.