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Figure 1 | Respiratory Research

Figure 1

From: Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant

Figure 1

Effect ofS. aureussupernatant on cell death. Cell death induced by S. aureus supernatant. (A) Propidium iodide fluorescent probe (red staining) was used to visualize the dead cells and syto 9 fluorescent probe (green staining) was used to visualize all the cells. The number of dead cells was increased in presence of 10% or 20% of S. aureus supernatant. (B) Time-dependent increase in fluorescence intensity of propidium iodide in presence of the different concentrations of S. aureus supernatant. (C) Fluorescence intensity of the propidium iodide probe after 5 h of incubation with the different concentrations of S. aureus supernatant. The increase in fluorescence was significant when the cells were incubated with 10% or 20% of S. aureus supernatant (*, p < 0.05; data represent the mean ± SEM of 3 different experiments). In parallel, the MTT technique showed the number of living cells. The decrease of OD was significant when the cells were incubated with 10% or 20% of S. aureus supernatant (**, p < 0.01; data represent the mean ± SEM of 8 different wells).

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