Figure 1
From: Divergent expression of claudin -1, -3, -4, -5 and -7 in developing human lung
Immunohistochemical staining for claudins 1, 3, 4, 5 and 7 in pseudoglandular period i.e. in weeks 12-16 in developing human lung. 1A. Epithelium of a bronchus is positive for claudin-1 (arrow). The short arrow is indicating the cartilage of the bronchus. Scale bar = 80 μm. 1B. Epithelial cells of developing bronchioles are positive for claudin-3 (arrows). Scale bar = 80 μm. 1C. Strong positivity for claudin-3 is seen in the epithelial cells of a bronchiole. Scale bar = 25 μm. 1D-1E. Epithelium of bronchioles is strongly positive for claudin-4 (arrows). Scale bar = 80 μm in Fig 1D, scale bar = 25 μm in Fig 1E. 1F. Endothelial (short arrow) and epithelial cells (arrow) of bronchial epithelium are positive for claudin-5. Scale bar = 80 μm. 1G. Epithelium of bronchi and developing airways are positive for claudin-7 (arrow). Scale bar = 80 μm. 1H. Negative control in which the primary antibody has been substituted with non-immune rabbit mouse serum. Scale bar = 80 μm.