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Figure 2 | Respiratory Research

Figure 2

From: Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells

Figure 2

Kinetics of FM 1-43 uptake into cells. Delta was expressed as relative fluorescence units (RFUs) and represents the rate of FM 1-43 uptake used for the values shown in Table 2. Images: Fluorescence microscopy demonstrates intracellular FM 1-43 uptake by ATII cells after 30 min and 3 hours incubation. Note that PFC droplets are not present. Inset: Rate of increase intracellular fluorescence intensity of FM 1-43 after concentration-dependent ATII cell incubation with β-Escin. These experiments demonstrate the sensitivity of this assay with respect to membrane perturbations. Data of FM 1-43 cell uptake were expressed as relative fluorescence units (RFUs).

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