Table 1 The primer sequence and PCR annealing temperature of quantitative PCR.
gene | Forward primer | Reverse primer | Annealing Tm (°C) |
|---|---|---|---|
COX1 | 5'-gcttcgtgaacataaccg-3' | 5'-ggatgccagtgatagagatg-3' | 58 |
COX2 | 5'-gtgcctggtctgatgatg-3' | 5'-aatgcggttctgatactgg-3' | 58.4 |
HPRT | 5'-caggccagactttgttggat-3' | 5'-ttgcgctcatcttaggcttt-3' | 58 |