Figure 5

CSE- and LPS-induced proliferation is dependent on ERK 1/2 and p38 MAP kinase. Serum-deprived BTSM cells were treated 2 times (1 h, day 0 and day 2) with CSE (15%) or 4 days continuously with LPS (1 μg/ml) in the absence or presence of the MEK inhibitor U0126 (3 μM) and the p38 MAP kinase inhibitor SB 203580 (10 μM) (panels A and B), as well as in the absence or presence of the MEK inhibitor PD 98059 (30 μM) and the p38 MAP kinase inhibitor SB 239063 (10 μM) (panels C and D). Cells were counted in duplicate on day 4, using a hemocytometer. Data represent means ± S.E.M. of 4-7 experiments. *P < 0.05, **P < 0.01 vs untreated control, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs CSE or LPS treatment in the absence of inhibitor.