Figure 1
From: Cigarette smoke and lipopolysaccharide induce a proliferative airway smooth muscle phenotype
CSE and LPS induce BTSM cell proliferation. Subconfluent, serum-deprived BTSM cells were treated with increasing concentrations of CSE for 1 h (A) or increasing concentrations of LPS for 28 h (B). [3H]-Thymidine incorporation was determined 28 h after stimulation as described under methods. Data represent means ± S.E.M. of 5-7 experiments, each performed in triplicate. (C) Serum-deprived BTSM cells were treated 2 times (1 h, day 0 and day 2) with CSE (15%) or 4 days with LPS (1 μg/ml) or PDGF (10 ng/ml). Cells were counted in duplicate on day 4, using a hemocytometer. Data represent means ± S.E.M. of 5-8 experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs control