Figure 1

COPD tissue contains increased numbers of CD83+ cells and CD207 (Langerin) mRNA levels compared to control tissue. A) Quantitative total tissue expression of CD1a+ cells identified in whole tissue slides from 8 controls and 16 COPD subjects. The values on the vertical axis refer to the area of positive staining relative to the entire scanned tissue biopsy. Graph shows mean and SEM of all data points. NS = not significant. B) Quantitative total tissue expression of CD83+ cells in 8 controls and 16 COPD subjects. The values on the vertical axis refer to the area of positive staining relative to the entire scanned tissue biopsy. Graph shows mean and SEM of all data points. C) Increased CD207 mRNA expression in total lung tissue from patients with COPD (n = 27) compared with controls without COPD (n = 7). Relative CD207 mRNA expression is shown as the ratio of the number of transcripts for CD207 to the number of transcripts for the housekeeping gene GAPDH. Graph shows mean and SEM of all data points. D) Representative images demonstrating the dendritic cells with dark brown membranous positivity on CD1a (left panel) and CD83 (right panel) stainings. Right panel; 58-year old ex-smoker with FEV1 63% predicted. Left panel; 78-year old ex-smoker with FEV1 60% predicted.