Table 1 Primers used for qRT-PCR
From: Partial pulmonary embolization disrupts alveolarization in fetal sheep
Gene of interest | Accession #, Species | Region amplified (nt#) | Primers 5'-3' F: Forward R: Reverse | Annealing temp. (°C) | ||
|---|---|---|---|---|---|---|
VEGF-A | AF071015.1, Ovis aries | 264-338 | F: CGAAAGTCTGGAGTGTGTGC R: TATGTGCTGGCTTTGGTGAG | 60 | ||
Flk-1 | AF534634, Ovis aries | 385-462 | F: CCCAATCAGAGACCCACG R: GCCATCCTGTTGAGCGTTA | 60 | ||
PDGF-A | NM_001075231.1, Bos taurus | 784-856 | F: ATGGCGTGTTACATTCCTA R: TTCACGGAGGAGAACAAAGA | 59 | ||
PDGF-Rα | XM_590921, Bos taurus | 213-276 | F: GCAGGAGATCAGAGTGGAGA R: TGAAAGCTGGCAGAGGATTA | 59 | ||
18S | X01117, Rattus norvegicus | 1495-1673 | F: GTCTGTGATGCCCTTAGATGTC R: AAGCTTATGACCCGCACTTAC | 59-60 | ||