Figure 2From: Partial pulmonary embolization disrupts alveolarization in fetal sheepLocalization and relative abundance of elastin and density of secondary septal crests in control and embolised fetal lung tissue. Light micrographs depicting the localization of elastin (purple-black) staining in control (A), 1d PPE + 15d (B), and 5d PPE + 16d (C) lung tissue. Nuclei and cytoplasm are counterstained yellow. The arrows in A-C indicate secondary septal crests and the arrow-heads indicate deposition of elastin throughout the primary septal wall and around the base of alveoli in PPE fetuses (B-C). The scale bar in (C) represents 10 μm in images A-C and the scale bar in F represents 10 μm in images D-F. The relative abundance of elastin (G) was decreased in 1d PPE + 15d and 5d PPE + 16d fetuses relative to control fetuses (* p < 0.05). The density of secondary septal crests in the lung parenchyma (H) of 1d PPE + 15d fetuses was lower than in control fetuses (*p < 0.001) and lower in 5d PPE + 16d fetuses relative to 1d PPE + 15d fetuses and control fetuses (**p < 0.05).Back to article page