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Figure 6 | Respiratory Research

Figure 6

From: Expression, regulation and clinical significance of soluble and membrane CD14 receptors in pediatric inflammatory lung diseases

Figure 6

In vitrostimulation of PBMCs. sCD14 levels in cell-culture supernatants were quantified in duplicates by ELISA. * p < 0.05 vs medium treatment. PBMCs (2 × 106) in RPMI medium were stimulated with PMA (10 ng/ml), fMLP (100 ng/ml) or the TLR ligands CpG-DNA (100 μg/ml), zymosan (50 μg/ml), PGN (1 μg/ml), Pam3CSK4 (1 μg/ml), flagellin (1 μg/ml) or LPS (100 ng/ml) for 1 hour (A) or 40 hours (B) at 37°C.

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