Induction of antioxidant genes is attenuated in the lungs ofNrf2-/-mice. (A) The concentration of 8-isoprostane, an oxidative stress marker, in the lungs of WT mice (open bars) or Nrf2-/- mice (solid bars) before, and 1 day and 3 days after bleomycin administration. n = 4 in each group. (B-F) The expression of GST-P1 (B), GCLC (C), NQO1 (D), HO-1 (E), and PrxI(F) mRNAs in the lungs of WT mice (open bars) or Nrf2-/- mice (solid bars) before, and 1 day (Day 1) and 3 days (Day 3) after bleomycin administration. GAPDH was used as an internal control. n = 3 in each group. *Significant difference (p < 0.05) between WT mice and Nrf2-/- mice.