A) Fold changes inIRAK3gene expression in healthy lungs after 4 hours of spontaneous breathing (non-ventilated) or mechanical ventilation with 6 ml/kg and 20 ml/kg. Bars represent the median fold-increase compared to non-ventilated animals. (*) p = 0.001 vs. non-ventilated animals. B) Representative blots from individual animals showing changes of IRAK-3 protein levels in lungs after 4 hours of mechanical ventilation with low or high V
. Histograms represent mean densitometric values showing IRAK-3 protein levels from all animals in each group (n = 6 animals per group). Data are reported as means ± SD and were obtained from 6 independent experiments. (*) p = 0.001 vs. non-ventilated animals. VT = tidal volume.