Figure 3
Effects of V T on protein levels in the lungs for TLR2, TLR4, and IκBα, analyzed by Western blotting in animals ventilated with low or high tidal volume for 4 h. (*) p < 0.05 vs. non-ventilated animals, (**) p < 0.01 vs. non-ventilated animals, (***) p < 0.001 vs. non-ventilated animals, t p < 0.001 vs. animals ventilated with low VT, τ p < 0.01 vs. animals ventilated with low VT. IMR-90 cell line was used as positive control for TLR2 protein levels. Note that these antibodies react with mouse, rat, and human epitopes. Data are reported as mean ± SD and were obtained from 6 animals in each group. VT = tidal volume.