Figure 3
Regulation of TGF-β1-induced E-cadherin expression by PPARγ ligands. (A) Effect of TGF-β1 on expression of epithelial marker E-cad (n = 4). (B) Effect of RGZ on regulation of E-cad expression by TGF-β1 (n = 6). (C, D) Effect of the PPARγ antagonist GW9662 (10 μM) on regulation of TGF-β1-mediated E-cad expression by RGZ (n = 4) and CGZ (n = 4). (E) Western blot showing the effect of GW9662 on RGZ-mediated inhibition of TGF-β1 effect on E-cad expression (representative of n = 4 experiments). Cell lysates were prepared from A549 cells pre-incubated with vehicle, RGZ or CGZ with or without GW9662 for 1 hr, stimulated with TGF-β1 at the concentrations indicated for 72 hr. Densitometric analysis values for band intensities from each Western blot were normalised to β-actin and expressed as a percentage of basal levels. Each point represents mean ± s.e.m. * P < 0.05, compared with basal (A) TGF-β1 (B-D).