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Figure 5 | Respiratory Research

Figure 5

From: Immunomodulatory strategies prevent the development of autoimmune emphysema

Figure 5

Effects of Immunomodulatory Strategies on Rat Lung Cytokine levels and AECA production. (A) Levels of IL-α, VEGF and CNTF in whole lung tissue homogenates from PBS-treated controls, HUVEC-immunized and pristane-pretreated/HUVEC-immunized rats measured by Quantibody Rat-1 Array. *p < 0.01 when compared to the untreated HUVEC-immunized rats. (B) Antibody production in HUVEC-immunized rats. HUVEC protein extracts were separated on 4-12% gradient Bis-Tris gel and WB was performed using serum (at 1:1000 dilution) from HUVEC-immunized (H) rats (lanes 1 and 2); intrathymically HUVEC-injected and HUVEC-immunized (H-T-H) rats (lanes 3 and 4); pristane pre-treated/HUVEC-immunized (H-P) animals (lanes 5 and 6); and pre-immune serum (lanes 7-10).

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