Figure 6

STIM1 knockdown abolished SOC currents. Na⁺ concentration in the electrode solution has been increased to 40 mM while Na⁺ concentration in the extracellular solution remains the same (140 mM) A)Time course of SOC current density (at -80 mV) in cells transfected with negative control siRNA (squares, n = 17) or STIM1 siRNA (diamonds, n = 17). B) Bar chart illustrating peak current density of SOC current in control siRNA transfected cells compared with cells transfected with STIM1 siRNA (mean data ± SEM, **p < 0.01). C) Representative traces of I-V relationships of SOC currents. Numbers 1-4 correspond to time points on time course chart A. D) Time course of NCX1 outwardly rectifying current density (at +80 mV) in cells transfected with negative control siRNA (diamonds, n = 17) or STIM1 siRNA (squares, n = 17). E) Bar chart illustrating peak current density of NCX1 outwardly rectifying current in control siRNA transfected cells compared with cells transfected with STIM1 siRNA; mean data ± SEM, **p < 0.01. F) Representative traces of I-V relationships of NCX1 outwardly rectifying currents.