Figure 2

Induction of KB-R7943 sensitive NCX1 outwardly rectifying currents under basal conditions. (A)Time course of subtracted outwardly rectifying current density (+80 mV), each point represents mean data; 140 mM extracellular Na⁺ (squares, n = 12) and 5 mM Na⁺ (diamonds, n = 15). (B)Time course of subtracted current density showing control cells (diamonds, n = 15) compared with addition of KB-R7943 (squares, n = 6). (C) A bar chart illustrating peak current density of these outwardly rectifying currents (mean data ± SEM *p < 0.05, **p < 0.01) (D) Raw values (non-subtracted) for current-voltage (I-V) relationships at 0 extracellular Ca²⁺, following application of 2 mM extracellular Ca²⁺ and reduced Na+ (5 mM) with or without KB-R7943. Note that in panel 2A and 2B baseline currents (zero Ca²⁺) have been subtracted from all traces. (E) Raw values (non-subtracted) for current-voltage (I-V) relationships at 0 extracellular Ca²⁺, following addition of 2 mM extracellular Ca²⁺ and reduced Na+ (5 mM) at time points of 41 seconds (peak current) and 120 seconds (end point).