Figure 3

Sarcoidosis CD8+ Th1 responses in BAL to multiple mycobacterial antigens. BAL from sarcoidosis subjects, PPD- diseased controls and non-Tuberculosis Mycobacterial (NTM) infection subjects were stimulated with neoantigen (KLH), ESAT-6, katG, Ag85A, sodA, HSP and SEB (positive control), then intracellular cytokine staining for IFN-γ was performed. (A) Gating strategy and representative flow data for CD8+ T cell IFN-γ responses. (B) The number of Sarcoidosis, PPD- and NTM subjects that did (red) or did not (blue) respond to stimulation with mycobacteria peptides. CD8+ T cell response was defined as positive when the frequency of recognition was at least twice background fluorescence and greater than 0.5%.