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Figure 5 | Respiratory Research

Figure 5

From: Legionella pneumophila induces cathepsin B-dependent necrotic cell death with releasing high mobility group box1 in macrophages

Figure 5

Cathepsin B is involved in L. pneumophila -induced murine macrophage death (A) Macrophages derived from the bronchoalveolar or peritoneal lavage fluid of A/J mice were stimulated with an MOI 500 of L. pneumophila for 6 h followed by staining with Hoechst 33342 and PI. The PI-positive cells (red) represent necrotic cells. L. pneumophila infection increased, and pretreatment with CA074Me decreased, the number of PI-positive cells. The scale bar represents 75 μm. (B) LDH release 6 h after L. pneumophila-infection in peritoneal macrophages. CA074Me pretreatment had an inhibitory effect on L. pneumophila-induced cytotoxicity. Values are expressed as means ± SD. ** indicates p < 0.01.

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Respiratory Research

ISSN: 1465-993X

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