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Figure 4 | Respiratory Research

Figure 4

From: Legionella pneumophila induces cathepsin B-dependent necrotic cell death with releasing high mobility group box1 in macrophages

Figure 4

L. pneumophila -induced high activation of cathepsin B is involved in PARP cleavage and cell death. (A) The activity of cathepsin B in cell lysates after L. pneumophila (MOI of 500) or MG132 treatment was measured. L. pneumophila rapidly and strongly enhanced cathepsin B activation. In contrast, MG132 gradually activated cathepsin B over the time of assay. Data are representative of three independent experiments. (B) Effects of CA074Me and a pan-caspase inhibitor (zVADfmk, 100 μM) on PARP cleavage after L. pneumophila or MG132 treatment at 2 h, assessed by western blot. L. pneumophila-induced PARP cleavage was attenuated by CA074Me but not by zVADfmk. In contrast, zVADfmk showed mild inhibition of MG132-induced PARP cleavage. Cont; control, zV; zVADfmk, CA; CA074Me. (C) Effects of CA074Me and zVADfmk on the cytotoxicity of L. pneumophila-treated cells. CA074Me, but not zVADfmk, significantly decreased L. pneumophila-induced LDH. Values are expressed as means ± SD. ** indicates p < 0.01.

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Respiratory Research

ISSN: 1465-993X

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