Figure 3From: Legionella pneumophila induces cathepsin B-dependent necrotic cell death with releasing high mobility group box1 in macrophages Cathepsin B regulates necrosis and apoptosis. (A) Classification of L. pneumophila-infected cells 6 h after stimulation based on morphological features. The percentage of necrotic cells was significantly decreased by pretreatment with CA074. (B) Annexin V and PI stained cells were evaluated by flow cytometry analysis 6 h after stimulation with L. pneumophila. The percentage of stained cells is indicated at the bottom of each panel. (C) Morphological classification of MG132-treated cells. MG132 induced-apoptotic cells were inhibited by pretreatment with CA074Me. (D) Flow cytometric analysis of MG132-treated cells stained with Annexin V and PI at 6 h. Values are expressed as means ± SD. ** indicates p < 0.01.Back to article page