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Figure 2 | Respiratory Research

Figure 2

From: Legionella pneumophila induces cathepsin B-dependent necrotic cell death with releasing high mobility group box1 in macrophages

Figure 2

L. pneumophila -induced THP-1 cell death is cathepsin B-dependent. (A) Western blot analysis of pro-cathepsin B expression following stimulation with an MOI of 500 of L. pneumonia for the indicated times. (B) The effect of the specific cathepsin B inhibitor, CA074Me, on the L. pneumophila -induced LDH. CA074Me inhibited L. pneumophila-induced cytotoxicity at concentrations greater than 50 μM. (C) The microscopic findings after pretreatment with CA074Me (100 μM) on L. pneumophila-induced necrotic cells. The top scale bar represents 50 μm and the bottom represents 20 μm. (D) The effect of CA074 pretreatment on L. pneumophila-induced elevation of HMGB-1. Error bars in (B) and (D) represent means ± SD. ** indicates p < 0.01.

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Respiratory Research

ISSN: 1465-993X

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