Figure 5

IFN-γ reduces cathepsin H but not ABCA3. Adult human ATII cells cultured on Matrigel and rat-tail collagen in DMEM containing 5% heat inactivated human serum with 2 d TGFα followed by 4 d KGF with 20 ng/ml IL-13 or with 100 ng/ml IFN-γ for the final 4 days. Panel A shows representative active form of cathepsin H protein levels by immunoblotting and protein levels by immunoblotting for the active form cathepsin H normalized by GAPDH from 6 different donors, which are analyzed by NIH Image. Lane 1: day 0 control, Lane 2: empty, Lane 3: 2 d 10 ng/ml TGFα + 4 d 10 ng/ml KGF, Lane 4: 2 d 10 ng/ml TGFα + 4 d 10 ng/ml KGF with 4 d 20 ng/ml IL-13, Lane 5: 2 d 10 ng/ml TGFα + 4 d 10 ng/ml KGF with 4 d 100 ng/ml IFN-γ. White bar: without IL-13 and IFN-γ, black bar: with 20 ng/ml IL-13 for 4 d, gray bar: with 100 ng/ml IFN-γ for 4 d. *: p < 0.05 v.s. without IL-13 and IFN-γ. Panel B shows representative ABCA3 protein levels by immunoblotting and protein levels by immunoblotting for ABCA3 normalized by GAPDH from 6 different donors, which are analyzed by NIH Image. Lane order is same as in Panel A. White bar: without IL-13 and IFN-γ, black bar: with 20 ng/ml IL-13 for 4 d, gray bar: with 100 ng/ml IFN-γ for 4 d. *: p < 0.05 v.s. without IL-13 and IFN-γ.