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Figure 3 | Respiratory Research

Figure 3

From: The effect of interleukin-13 (IL-13) and interferon-γ (IFN-γ) on expression of surfactant proteins in adult human alveolar type II cells in vitro

Figure 3

IL-13 and IFN-γ alter surfactant protein mRNA levels. Adult human ATII cells were cultured on Matrigel and rat-tail collagen coated inserts in DMEM containing 5% heat inactivated human serum with 2 d TGFα followed by 4 d KGF. Panel A shows mRNA data from ATII cells cultured with 20 ng/ml IL-13 (black) for 2, 4 or 6 d. mRNA levels for surfactant proteins were measured by quantitative real-time PCR and normalized to the constitutive probe 36B4 (n = 6). *: p < 0.05 v.s without IL-13. Panel B shows mRNA data from ATII cells cultured with 100 ng/ml IFN-γ (gray) for 4 d. mRNA levels for surfactant proteins were normalized to the constitutive probe 36B4 by quantitative real-time PCR (n = 6). *: p < 0.05 v.s without IFN-γ

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