Figure 5
From: SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling
Effects NF- κ B pathway on SPLUNC1 production in primary mouse tracheal epithelial cells. Tracheal epithelial cells from CC10-tetracycline-inducible CA-IKKβ (CC10-CA-IKKβ) transgene positive and negative C57BL/6 mice were isolated and cultured under the air-liquid interface conditions for 10 days. Cells were then treated with water (H2O) or doxycycline (Dox) for 48 hrs. SPLUNC1 mRNA in epithelial cells was quantified by using real-time PCR. As compared to H2O treatment, Dox significantly increased SPLUNC1 mRNA levels in epithelial cells from CC10-CA-IKKβ transgene positive (Tg+), but not from transgene negative (Tg-) mice. Data are expressed as means ± SEM (N = 4 replicates).