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Figure 3 | Respiratory Research

Figure 3

From: SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling

Figure 3

TLR2 dependence of SPLUNC1 production in normal human bronchial epithelial cells (NHBE). NHBE (N = 3) were transduced with firefly luciferase short hairpin RNA (Luc shRNA, control) or TLR2 short hairpin RNA (TLR2 shRNA). Thereafter, cells were seeded onto 12-well transwell plates for air-liquid interface (ALI) culture for 10 days, and were then treated with or without Mycoplasma pneumoniae (Mp, 10 cfu/cell) or Pam3CSK4 (1 μg/ml) for 48 hrs. Apical supernatants were collected for SPLUNC1 protein measurement using an ELISA. The paired t test (for normally distributed data under Luc shRNA conditions) or Wilcoxon matched pairs test (for non-parametric data under TLR2 shRNA conditions) was used to analyze the treatment effect of Mp or Pam3CSK4 (Pam3) on SPLUNC1 protein levels. While Mp and Pam3CSK4 increased SPLUNC1 in NHBE transduced with Luc shRNA, they failed to do so in TLR2 shRNA-transduced cells.

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