Figure 1
From: SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling
A dose response of SPLUNC1 production in NCI-H292 cells. Cells were cultured in 6-well plates with Mycoplasma pneumoniae (Mp, 1 - 10 cfu/cell) or a TLR2 agonist (Pam3CSK4, 10 - 1000 ng/ml) for 48 hrs, followed by collection of supernatants and cell lysates for SPLUNC1 mRNA and protein measurements, respectively. Data are expressed as means ± SEM (N = 6 replicates). (A) Mp increased SPLUNC1 protein levels in cell supernatants in a dose-dependent manner. (B) Mp increased SPLUNC1 mRNA expression at 1 and 5 cfu/cell. (C) Pam3CSK4 at 100 and 1000 ng/ml significantly augmented SPLUNC1 protein levels in cell supernatants. ND = Not detectable. (D) Pam3CSK4 enhanced SPLUNC1 mRNA expression in a dose-dependent fashion. (E) Western blot analysis of intracellular SPLUNC1 protein and β-actin (a protein loading control) in Mp (10 cfu/cell) and Pam3CSK4 (100 ng/ml)-treated cells using the Odyssey Imaging System (two color detection). Both Mp and Pam3CSK4 increased SPLUNC1 protein.