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Figure 3 | Respiratory Research

Figure 3

From: Chronic OVA allergen challenged Siglec-F deficient mice have increased mucus, remodeling, and epithelial Siglec-F ligands which are up-regulated by IL-4 and IL-13

Figure 3

Levels of peribronchial fibrosis, TGF-β1+ cells, and LTC4 levels in Siglec-F deficient vs WT mice. Different groups of Siglec-F deficient or WT mice were subjected to chronic OVA challenge. Non-OVA challenged mice served as a control. Levels of peribronchial fibrosis were quantitated by assaying collagen levels in lungs (Fig 3A), as well as by quantitating the area of peribronchial trichrome staining by image analysis (Fig 3B). Levels of mediators of lung fibrosis were assessed by quantitating the number of peribronchial cells immunostaining positive for TGF-β1 (Fig 3C) as well as levels of LTC4 in BAL (Fig 3D) by Elisa. Chronic OVA challenge in WT mice induced a significant increase in lung collagen (p < 0.0001*)(Fig 3A), and the area of peribronchial trichrome staining (p < 0.0001*)(Fig 3B), the number of peribronchial TGF-β1+ cells (p < 0.0001*)(Fig 3C), and levels of BAL LTC4 (p < 0.02*)(WT no OVA vs WT OVA). Levels of lung collagen were significantly increased in OVA challenged Siglec-F deficient mice compared to WT mice challenged with OVA (p < 0.002#)(WT OVA vs Siglec-F OVA)(Fig 3A), as was the area of peribronchial trichrome staining (p < 0.01#)(Fig 3B), and the number of peribronchial TGF-β1+ cells (p < 0.001)(Fig 3C)(n = 16 mice/group).

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