Figure 2

Levels of mucus expression in Siglec-F deficient vs WT mice. Different groups of Siglec-F deficient or WT mice were subjected to chronic OVA challenge. Non-OVA challenged mice served as a control. The level of mucus expression was quantitated in lung sections by PAS staining using a light microscope objective at 20× (Fig 2 A-E). Chronic OVA challenge in WT mice induced a significant increase in the number of PAS+ mucus cells (p = 0.0001*)(WT no OVA vs WT OVA)(Fig 2A; Fig 2B vs Fig 2D). Levels of mucus expression were significantly increased in OVA challenged Siglec-F deficient mice compared to OVA challenged WT mice (p = 0.0001^#)(Fig 2A; Fig 2E vs Fig 2D)(n = 16 mice/group).