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Figure 3 | Respiratory Research

Figure 3

From: Inhibitors of inflammation and endogenous surfactant pool size as modulators of lung injury with initiation of ventilation in preterm sheep

Figure 3

Localization of HSP70 mRNA, Egr-1 protein and MCP-1 protein. HSP70 in situ localization demonstrates signal in bronchial epithelium in controls (A), which is lost in ventilate lambs (B, C). Induction of HSP70 mRNA in smooth muscle surrounding airways is seen in lambs with < 5 μmol/kg Sat PC (B) and lambs with >5 μmol/kg (C). Egr-1 protein is not in airways of control lambs (D) but Egr-1 protein staining is qualitatively increased in lambs ventilated with < 5 μmol/kg Sat PC (E) verses lambs with > 5 μmol/kg (F). Compared to MCP-1 in controls (G), MCP-1 protein is localized to the mesenchyme surrounding the medium sized airways and to the inflammatory cells in lambs ventilated with < 5 μmol/kg Sat PC (H) with minimal staining in animals with > 5 μmol/kg (I). Scale bar = 50 μm, insert 25 μm.

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