Figure 3
From: Phosphodiesterase 6 subunits are expressed and altered in idiopathic pulmonary fibrosis
Cellular and sub-cellular localization of the PDE6 subunits. (A) Immunohistochemical stainings were performed on serial tissue sections of donor (upper row) and IPF (bottom row) lungs. PDE6A, PDE6B, PDE6D and PDE6G/H were co-stained with pro-SPC, a marker specific for ATII cells. PDE6A immunoreactivity was recognized in the cytoplasm and membrane of ATII cells, PDE6B immunoreactivity was recognized in the nuclei, PDE6D immunoreactivity in the cytoplasm and PDE6G/H immunoreactivity in the membrane of ATII cells. The red and dark brown color is indicative of immunoreactivity. Tissue slides were counterstained with hematoxylin (blue color). Isotype control stands for rabbit serum reaction and null control stands for no antibody reaction, magnification 630×. Arrows indicate stained cells. (B) PDE6 mRNA expression in human primary donor and IPF-derived ATII cells. Primary human ATII cells were isolated from whole lung tissue of donor and IPF patients as described in Material and Methods. The mRNA levels of PDE6A, PDE6B, PDE6D, PDE6G and PDE6H were analyzed by qRT-PCR. Results are derived from 3 different donor and IPF patients. Each reaction was performed in quadriplicates. Data were present as mean ± S.E, *P < 0.01 versus donor ATII cells.