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Figure 4 | Respiratory Research

Figure 4

From: DMF inhibits PDGF-BB induced airway smooth muscle cell proliferation through induction of heme-oxygenase-1

Figure 4

GSH reverses the effects of DMF on p38 MAPK phosphorylation, on HO-1 expression and on ASMC proliferation. (A) a representative immuno-blots of the reversing effect of GSH on DMF- and PDGF-BB induced of p38 MAPK phosphorylation in ASMC at 30 min. Similar results were obtained in three cell lines.. (B) a representative immuno-blot of the reversing effect of GSH on the DMF-induced HO-1 expression at 24 h; similar results were obtained in three additional cell lines.. (C) a counteractive effect of GSH on DMF dependent inhibition of ASMC proliferation. Similar results were obtained in four cell lines. Data represents mean ± SEM (unpaired student's t-test). "V" indicates the drug's vehicle 0.05% DMSO. (D) down regulation of HO-1 by a respective siRNAs counteracted the anti-proliferative effect of DMF. Data represents the mean ± SEM of 9 independent experiments performed in 3 ASMC lines. Statistics have been calculated by Wilcoxon-Mann-Whitney U-test.

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