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Figure 6 | Respiratory Research

Figure 6

From: Decreased Toll-like receptor 8 expression and lower TNF-alpha synthesis in infants with acute RSV infection

Figure 6

Decreased TNF-alpha levels in monocytes from RSV-infected infants. Fresh PBMC from healthy adults (n = 10), age-matched healthy infants (n = 10) and infants with acute RSV infection (n = 10) were stimulated with 5 μg/mL CL075 for 6 h in the presence of brefeldin A. Intracellular TNF-α was stained with anti-TNF-α APC-conjugated antibody in CD68-positive monocytes upon permeabilization. Non-parametric Kruskal-Wallis ANOVA test was acquired to confirm statistical significance and Mann-Whitney U-test was applied for intergroup analysis.

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