Figure 2

Functional ability of monocytes to respond to specific TLR8 stimulation and produce TNF-alpha. The monocytes were stimulated with 5 μg/mL CL075 and 100 ng/mL LPS for 6 h. Brefeldin A was added to inhibit cytokine release in the supernatant and intracellular TNF-α was stained with anti-TNF-α APC-conjugated antibody in CD68-positive monocytes. In separate experiments PBMC were pretreated for 1 hour with chloroquine prior CL075 stimulation.