Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells

Table 2 Effect of the hNE inhibitor EPI-hNE4 on ΔI _sc in HNEC from control and CF patients.

	Control HNEC		CF HNEC
	Vehicle	EPI-hNE4 (100 μg/ml)	Vehicle	EPI-hNE4 (100 μg/ml)
ΔI _sc (μA/cm²)	0.5 ± 2.60	-0.9 ± 2.91	0.2 ± 2.15	-0.3 ± 2.52

Human nasal epithelial cells (HNEC) from control (non CF) and CF patients grown for 14 to 21 days on semi-permeable transwell filters were mounted in Ussing chamber and treated apically with either the hNE inhibitor EPI-hNE4 (final concentration 100 μg/ml) or vehicle. ΔI _sc represents the difference between final I _sc value at the end of experiment and basal I _sc value before apical addition of vehicle or EPI-hNE4 in control and CF HNEC. Results are expressed as means ± SE (n = 4-5 individual filters for each condition).

ISSN: 1465-993X