| | Control HNEC | CF HNEC |
|---|
PD (mV) | 36.8 ± 2.18 | 57 ± 2.23 *** |
R
te (Ω.cm2) | 969 ± 67.7 | 953 ± 48.1 |
I
sc (μA/cm2) | 41.8 ± 3.42 | 60.7 ± 3.19 ** |
- Human nasal epithelial cells (HNEC) from control (non CF) and CF patients were grown for 14 to 21 days on semi-permeable transwell filters until transepithelial resistance developed. Transwell filters were mounted in Ussing chamber for measurement of voltage (PD) and short-circuit current (I
sc). Transepithelial resistance (R
te) was calculated with Ohm's law from I
sc and PD. Results represent means ± SE of 25 individual filters from 9 separate cultures of non CF HNEC, and of 9 individual filters from 4 separate cultures of CF HNEC.
- **, ***: significantly different from corresponding value in control HNEC group (P < 0.01 and P < 0.001, respectively).
