Figure 1

Expression of tachykinin, tachykinin receptor and NEP mRNAs in human bronchi. RT-PCR product of the housekeeping gene HPRT used as normalization standard is also represented. Equal aliquots of each cDNA sample (human bronchi or human fetal brain positive control) were amplified for 40 PCR cycles with their respective specific primer pairs. Since TACR2 was not expressed in human fetal brain, another bronchi sample was used as positive control for this gene.