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Table 3 Antibodies used in the study.

From: Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration

Target

Immunogen

Host

Dilution

Source

nAChR α3

Synthetic peptide (aa 466-474 of human sequence)a

Rabbit

1:1600

Acris

nAChR α4

Synthetic peptide (620-627, human)a

Rabbit

1:800

Acris

nAChR α5

Synthetic peptide (460-468, human)a

Rabbit

1:1600

Acris

nAChR α7

Synthetic peptide (493-502, human)a

Rabbit

1:1000

Acris

nAChR α7

Native and denatured α7 subunit (380-400, chicken) and denatured α7 subunit from rat

Mouse, monoclonal, clone mAb 306

1:750

Sigma-Aldrich

nAChR α9

Synthetic peptides (81-97 and 115-128, rat)

Guinea-pig

1:1000

[44]

nAChR α10

Synthetic peptide (404-418, rat)a

Rabbit

1:2000

[17]

nAChR β2

Synthetic peptide (493-502, human)a

Rabbit

1:1600

Acris

nAChR β3

Synthetic peptide (450-458, human)a

Rabbit

1:800

Acris

nAChR β4

Synthetic peptide (490-498, human)a

Rabbit

1:3200

Acris

CD68-like

Rat spleen cells

Mouse, monoclonal, clone ED1

1:800

Serotec

pSTAT3 Tyr705

Synthetic phospho-peptide residues surrounding Tyr705 of mouse Stat3

Rabbit, monoclonal, clone D3A7

1:1000

Cell Signaling

pSTAT3 Ser727

Synthetic phospho-peptide residues surrounding Ser727 of mouse Stat3

Rabbit, monoclonal

1:1000

Cell Signaling

STAT3

Synthetic peptide corresponding to the sequence of mouse Stat3

Rabbit, monoclonal

1:1000

Cell Signaling

  1. Origin and regions of nAChR subunits sequences used for generation of the antibodies are provided in brackets. aPeptides available for preabsorption.