Figure 9From: Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentrationNicotine does not induce STAT-3 phosphorylation. BAL cells were stimulated with nicotine at 10-4 M, 10-5 M, and 10-6 M or GM-CSF at (100 ng/ml) for 0, 5, 15, 30 and 60 min, as indicated. Primary rabbit antibodies directed to phospo-STAT 3 (Tyr 705) or to total STAT 3 were used. Only GM-CSF induced phosphorylation of STAT3, as shown on the blot.Back to article page