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Figure 8 | Respiratory Research

Figure 8

From: Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration

Figure 8

Nicotinic receptors on mouse BAL cells. (A) RT-PCR, agarose gel electrophoresis. Mouse BAL cells consistently express mRNAs coding for α9, β2 and β4 subunits. Subunit α10 shows an interindividual expression pattern. The mRNA for α7 subunit was not found in BAL preparations, although it was easily detectable in brain homogenate, serving as a positive control. M = DNA marker, H2O = water control (B) Ratiometric [Ca2+]i recordings. Mouse BAL cells isolated from C57BL6N, α7 nAChR knockout (α7-/-) and littermate control animals (α7+/+) were stimulated with ATP (10-4 M) in the presence or absence (Hepes) of nicotine (10-4 M). Peak increases shown as mean ± SEM; cells taken from 6-9 coverslips for each experimental setup, p-values calculated by Mann-Whitney test.

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