Figure 6
Purinergic receptors on freshly isolated BAL cells. ATP-induced transients in [Ca2+]i are mainly dependent on intracellular stores. (A) BAL cells were stimulated with ATP (10-4 M) in the presence or absence of extracellular Ca2+. Sustained increase, but not initial rise in [Ca2+]i is dependent on extracellular Ca2+. Number of cells which reacted, and total number of measured cells are given in brackets. n.s. = not significant, ***P ≤ 0.001. (B) RT-PCR in rat BAL cells shows expression of P2Y1, P2Y2 and P2Y4 purinergic receptors.