Figure 2

Double-labeling immunofluorescence demonstrating the presence of the nAChR subunits α5, α9, α10 and β2 in ED1-positive BAL cells. A punctate fluorescence pattern for the α5 and α9 nAChR subunits was found in a subset of AM (inserts). Negative results were noted for α3 and α7 subunits in ED1-positive cells, despite occasional occurrence of α3 subunit immunoreactive ED1-negative cells (arrow). Bar: 20 μm, 10 μm in inserts.