Figure 4

Involvement of the IκBα/NF-κB pathway in IL-8 secretion induced by CSE, IL-1β and MCh. hASMc were stimulated with CSE (5%) (Figure 4A) or IL-1β (1 ng/mL) (Figure 4B) in the absence or presence of MCh (10 μM) and/or SC514 (50 μM) for 24 hours. Supernatants were analyzed for the presence of IL-8. Data represent means ± SE of 5 independent experiments each performed in duplicate. **p < 0.01 and ***p < 0.001 compared to basal; ^†p < 0.05, ^††p < 0.01 and ^†††p < 0.001 compared to the absence of SC514; ^$$p < 0.01 compared to CSE (One-way ANOVA followed by Newman-Keuls multiple comparisons test). (Figure 4C, 4 D and 4E) hASMc were stimulated with CSE (5%) (Figure 4C and 4D) or IL-1β (1 ng/mL) (Figure 4C and 4E) in the absence or presence of MCh for 60 min and 120 min (representative blots shown in Figure 4C) as indicated. IκBα degradation was determined by western blot and corrected for the expression of β-actin, which was used as a loading control. Data represent means ± SE of 9-10 experiments. *p < 0.05 and ***p < 0.001 compared to basal and ^††p < 0.01 compared the absence of MCh (Student's t-test for paired observations).